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KMID : 0379520170330010055
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한국독성학회지
2017 Volume.33 No. 1 p.55 ~ p.62
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Anti-Melanogenic Effect of Oenothera laciniata Methanol Extract in Melan-a Cells
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Kim Su-Eun
Lee Chae-Myoung
Kim Young-Chul
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Abstract
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We evaluated the antioxidant activity and anti-melanogenic effects of Oenothera laciniata methanol extract (OLME) in vitro by using melan-a cells. The total polyphenol and flavonoid content of OLME was 66.3 and 19.0 mg/g, respectively. The electron-donating ability, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity, and superoxide dismutase (SOD)-like activity of OLME (500 μg/mL) were 94.5%, 95.6%, and 63.6%, respectively. OLME and arbutin treatment at 50 μg/mL significantly decreased melanin content by 35.5% and 14.2%, respectively, compared to control (p < 0.05). OLME and arbutin treatment at 50 μg/mL significantly inhibited intra-cellular tyrosinase activity by 22.6% and 12.6%, respectively, compared to control (p < 0.05). OLME (50 μg/mL) significantly decreased tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor-M (MITF-M) mRNA expression by 57.1%, 67.3%, 99.0%, and 77.0%, respectively, compared to control (p < 0.05). Arbutin (50 μg/mL) significantly decreased tyrosinase, TRP-1, and TRP-2 mRNA expression by 24.2%, 42.9%, and 48.5%, respectively, compared to control (p < 0.05). However, arbutin (50 μg/mL) did not affect MITF-M mRNA expression. Taken together, OLME showed a good antioxidant activity and anti-melanogenic effect in melan-a cells that was superior to that of arbutin, a well-known skin-whitening agent. The potential mechanism underlying the anti-melanogenic effect of OLME was inhibition of tyrosinase activity and down-regulation of tyrosinase, TRP-1, TRP-2, and MITF-M mRNA expression.
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KEYWORD
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Anti-melanogenic effect, Antioxidant activity, Melan-a cells, Tyrosinase activity, Tyrosinase-related protein
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